Pham Anh Thuy Duong

Pham Anh Thuy Duong, MSc, is a highly experienced expert and researcher who has taken part in in-depth research projects and in developing and establishing specialized research laboratories and DNA/RNA-related testing procedures. In particular, she helped establish a laboratory and develop SARS-CoV-2 testing procedures at a major hospital.

Areas of Expertise: Immunology – Gene Technology – Cell Technology

Education, Certificates and Training:

University of Science, Vietnam National University, Hanoi (VNU)

• 2006, Bachelor of Biology – Very Good – Degree No. QC 041100

University of Greifswald, Germany; University of Science (VNU); Institute of Biotechnology

• 2008, Certificate of Completion of a Diploma-equivalent Program – Excellent

University of Science (VNU)

• 2009, Master’s degree in Biology – Degree No. QM 008403

University of Science (VNU), Carl Zeiss Micro-Imaging GMBH

• 2009, Certificate of Training in Fluorescence Microscopy Techniques for Protein and Cell Research

School of Science and Technology Management, Ministry of Science and Technology

• 2015, Certificate of Training in Laboratory Biosafety – No. 0281/2015/CC-TQL

Department of Science, Technology and Training, Ministry of Health

• 2017, Certificate of Training in Good Clinical Practice and Medical Ethics

BD Biosciences Vietnam

• 2017, Certificate of completion of BD FACSCanto^TM operation
• 2021, Certificate of completion of BD FACSCanto^TMII operation

Japan Science and Technology Agency

• 2019, Certificate of completion of the SAKURA Science Exchange Program – Member of the SAKURA Science Club.

Institute of Health Management Science, Vietnam Union of Science and Technology Associations

• 2025, Certificate of Continuing Training in Basic Medical Education – No. 7961/2025-B78

Hanoi Medical University, Ministry of Health

• 2025, Certificate of participation in continuing medical education in medical examination and treatment – No. 254358/A001/GCN-ĐHYHN-TTĐT

Professional Experience:

2007 – 2009 • Researcher • Biomedicine • Faculty of Biology, University of Science (VNU)

• Performed techniques involving DNA, RNA, proteins, microorganisms, and other biological materials
• Planned and designed experiments for scientific research projects
• Participated in practical classes and supervised students’ graduation theses
• Took part in laboratory management activities, including ordering supplies and maintaining records

2009 – 2015 • Research trainee • Immunology • Institute of Pathology, University of Würzburg, Germany

• Built up knowledge and practised techniques in molecular and cellular immunology.

2015 – 2016 • Researcher • Biomedicine • Faculty of Biology, University of Science (VNU)

• Helped prepare presentations, scientific reports and research proposals
• Performed statistical analysis of research results
• Took part in teaching and training.

2017 – 2018 • Gene Technology Specialist • Gene Technology • Vinmec Research Institute of Stem Cell and Gene Technology

• Studied ISO 15189 requirements to establish procedures for selected molecular biology tests, ensuring compliance with quality requirements and control policies
  
• Designed a GMP-oriented cell production laboratory and developed a GMP-oriented production process for a project on chimeric antigen receptor T cells
• Participated in designing a forensic laboratory and in forensic testing

2019 – 2021 • Molecular Biology Specialist • Molecular Biology • Laboratory Center, Military Hospital 175

• Developed and implemented molecular biology tests (genetics, cancer, microbiology)
• Established a testing laboratory and implemented SARS-CoV-2 testing

2021 – present • Senior Researcher • Stem Cells and Immunology • Mescells Research Institute

• Responsible for quality testing of mesenchymal stem cell products: establishing workflows, procedures, work instructions and forms
• Directly manages cell production, cell quality testing and laboratory operations to ensure cell production targets are met and products are supplied as required by clinical units (since Nov 2025)
• Develops, improves and optimizes procedures and work instructions relating to professional and non-professional activities (personnel, finance, services…)
• Establishes the system of quality standards for the cell production and quality testing laboratory in line with GMP-WHO and ISO 17025
• Leads research and development: proposing and carrying out institutional-level scientific research projects
• Compiles technical documents and lectures for the Institute’s CME training activities
• Provides professional training for the Institute’s researchers

Published Research and Scientific Reports:

1. Vo, T., Nguyen, T. N., Nguyen, T. T., Pham, A., Vuong, D. L., Ta, V. T., & Ho, V. S. (2022). SHOX2 methylation in Vietnamese patients with lung cancer. Molecular biology reports, 49(5), 3413–3421. https://doi.org/10.1007/s11033-022-07172-z
2. Thuy Duong, P., The Tung, P., Thi Quynh Trang, T., Thu Phuong, L., & Lan, V. (2022). Development of a Standard Control for qMSP to Analyze the Methylation Status of LINE-1. VNU Journal Of Science: Medical And Pharmaceutical Sciences, 38(1). https://doi.org/10.25073/2588-1132/vnumps.4314
3. Duong, P., Trang, N., Ngan, N., & Lan, V. (2021). Opting for Local Region in SHOX2 Promoter as a DNA Methylation Biomarker for Lung Cancer Diagnosis. VNU Journal Of Science: Natural Sciences And Technology, 37(4). https://doi.org/10.25073/2588-1140/vnunst.5333
4. Pham DAT, Le SD, Doan TM, Luu PT, Nguyen UQ, Ho SV, Vo LTT. (2021) Standardization of DNA amount for bisulfite conversion for analyzing the methylation status of LINE-1 in lung cancer. PLoS ONE 16(8): e0256254. https://doi.org/10.1371/journal.pone.0256254
5. Lan, V., Son, H. V., Trang, V. L., Trang, N. T., Phuong, N. T., Toan, N. L., & Duong, P. (2018). Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers. Molecular medicine reports, 18(2), 2476–2484. https://doi.org/10.3892/mmr.2018.9182
6. Muhammad, K., Rudolf, R., Pham, D., Klein-Hessling, S., Takata, K., Matsushita, N., Ellenrieder, V., Kondo, E., & Serfling, E. (2018). Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation. Frontiers in immunology, 9, 32. https://doi.org/10.3389/fimmu.2018.00032
7. Duong, P., Thu, T., & Lan, V. (2016). Methylation of miRNA-34a Promoter: a Preliminary Study in Vietnamese Breast Cancer Patients. VNU Journal Of Science: Natural Sciences And Technology, 32(1S).
8. Alrefai, H., Muhammad, K., Rudolf, R., Pham, D. A., Klein-Hessling, S., Patra, A. K., Avots, A., Bukur, V., Sahin, U., Tenzer, S., Goebeler, M., Kerstan, A., & Serfling, E. (2016). NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. Nature communications, 7, 11724. https://doi.org/10.1038/ncomms11724
9. Muhammad, K., Alrefai, H., Marienfeld, R., Pham, D. A., Murti, K., Patra, A. K., Avots, A., Bukur, V., Sahin, U., Kondo, E., Klein-Hessling, S., & Serfling, E. (2014). NF-κB factors control the induction of NFATc1 in B lymphocytes. European journal of immunology, 44(11), 3392–3402. https://doi.org/10.1002/eji.201444756
10. Lan, V. T., Ha, N. T., Uyen, N. Q., Duong, N. T., Huong, N. T., Thuan, T. B., Duong, P. A., & To, T. V. (2014). Standardization of the methylation‑specific PCR method for analyzing BRCA1 and ER methylation. Molecular medicine reports, 9(5), 1844–1850. https://doi.org/10.3892/mmr.2014.1990
11. Hock, M., Vaeth, M., Rudolf, R., Patra, A. K., Pham, D. A., Muhammad, K., Pusch, T., Bopp, T., Schmitt, E., Rost, R., Berberich-Siebelt, F., Tyrsin, D., Chuvpilo, S., Avots, A., Serfling, E., & Klein-Hessling, S. (2013). NFATc1 induction in peripheral T and B lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 190(5), 2345–2353. https://doi.org/10.4049/jimmunol.1201591
12. Bhattacharyya, S., Deb, J., Patra, A. K., Thuy Pham, D. A., Chen, W., Vaeth, M., Berberich-Siebelt, F., Klein-Hessling, S., Lamperti, E. D., Reifenberg, K., Jellusova, J., Schweizer, A., Nitschke, L., Leich, E., Rosenwald, A., Brunner, C., Engelmann, S., Bommhardt, U., Avots, A., Müller, M. R., … Serfling, E. (2011). NFATc1 affects mouse splenic B cell function by controlling the calcineurin–NFAT signaling network. The Journal of experimental medicine, 208(4), 823–839. https://doi.org/10.1084/jem.20100945
13. Lan, V. T., Loan, P. T., Duong, P. A., Thanh, l., Ha, N. T., & Thuan, T. B. (2012). Straightforward procedure for laboratory production of DNA ladder. Journal of nucleic acids, 2012, 254630. https://doi.org/10.1155/2012/254630
14. Hung, L., Loan, P., Duong, P., Lan, V. (2008). Study on production of quality DNA ladders. VNU Journal of Science, Nat., Sci., & Tech., 24, No.2S: 352 – 356.
15. Anh, D., Duong, P., Lan, V. (2008). Improving the method for detecting CYP21 gene mutation in patients with congenital hyperplasia syndrome. VNU Journal of Science, Nat., Sci., & Tech., 24, No.2S: 320 – 324.\
16. Duong, P., Thuan, T., Lan, V. (2007) Effects of Ganoderma lucidum extract on growth of Escherichia coli. VNU Journal of Science, Nat., Sci., & Tech., 23, No.1S:146 – 150.
17. Huy, N., Duong, P., Huong, P., Nghia, P. (2006) Inhibitory effects of Syzygium resinosum Gagnep extracts on caries-inducing properties of Streptococcus mutans. VNU Journal of Science, Nat., Sci., & Tech., 22, No.3CAP:134 – 139.
18. Huy, N., Duong, P., Nghia, P. (2005) Inhibition of acid production by Streptococcus mutans of some medicinal plant extracts. VNU Journal of Science, Nat., Sci., & Tech., 21, No.4AP:161-167.